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Synergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment.

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Izquierdo-Barba, Isabel and Santos-Ruiz, L and Becerra, J and Feito Castellano, María José and Fernandez-Villa, D and Serrano, M.C and Diaz-Gúemes, I and Fernandez-Tome, B and Enciso, S and Sanchez-Margallo, F.M and Monopoli, D and Alfonso, H and Portolés Pérez, María Teresa and Arcos Navarrete, Daniel and Vallet Regí, María (2018) Synergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment. Acta Biomaterialia . ISSN 1742-7061 (In Press)

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Abstract

The osteogenic and angiogenic responses to metal macroporous scaffolds coated with silicon substituted hydroxyapatite (SiHA) and decorated with vascular endothelial growth factor (VEGF) have been evaluated in vitro and in vivo. Ti6Al4V-ELI scaffolds were prepared by electron beam melting and subsequently coated with Ca10(PO4)5.6(SiO4)0.4(OH)1.6 following a dip coating method. In vitro studies demonstrated that SiHA stimulates the proliferation of MC3T3-E1 pre-osteoblastic cells, whereas the adsorption of VEGF stimulates the proliferation of EC2 mature endothelial cells. In vivo studies were carried out in an osteoporotic sheep model, evidencing that only the simultaneous presence of both components led to a significant increase of new tissue formation in osteoporotic bone.
STATEMENT OF SIGNIFICANCE
Reconstruction of bones after severe trauma or tumors extirpation is one of the most challenging tasks in the field of orthopedic surgery. This scenario is even more complicated in the case of osteoporotic patients, since their bone regeneration capability is decreased. In this work we present a porous implant that promotes bone regeneration even in osteoporotic bone. By coating the implant with an osteogenic bioceramics such as silicon substituted hydroxyapatite and subsequent adsorption of vascular endothelial growth factor, these implants stimulate the bone ingrowth when they are implanted in osteoporotic sheep.


Item Type:Article
Additional Information:

RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)
ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)
RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Bone regeneration, Silicon hydroxyapatite, VEGF, Osteoporosis, Scaffold
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:50139
Deposited On:23 Nov 2018 10:59
Last Modified:23 Nov 2018 13:03

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