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Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis

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Sijilmassi, Ouafa and López Alonso, José Manuel and Barrio Asensio, María del Carmen and Río Sevilla, del, Aurora (2019) Alteration of lens and retina textures from mice embryos with folic acid deficiency: image processing analysis. Graefe's Archive for Clinical and Experimental Ophthalmology, 257 (1). pp. 111-123. ISSN 0721-832X

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Official URL: https://doi.org/10.1007/s00417-018-4176-5




Abstract

Purpose: Folic acid (FA) is an essential vitamin for embryonic development. It plays particularly a critical role in RNA, DNA and protein synthesis. On the other hand, the collagen IV and laminin-1 are important proteins during embryonic development. This study was done to find if FA deficiency at a short and a long term in mothers could alter the tissue texture of retina and lens of the progeny.
Methods: Collagen IV and laminin-1 were localized by immunohistochemistry in the lens and retina of the FA-deficient embryos. To carry out the image processing, texture segmentation was performed through canny edge detection and Fourier transform (FT). We defined a parameter, the grain size, to describe the texture of the lens and retina. A bootstrap method to estimate the distribution and confidence intervals of the mean, standard deviation, skewness and kurtosis of the grain size has been developed.
Results: Analysis through image processing using Matlab showed changes in the grain size between control- and FA-deficient groups in both studied molecules. Measures of texture based on FT exhibited changes in the directionality and arrangements of type IV collagen and laminin-1.
Conclusions: Changes introduced by FA deficiency were visible in the short term (2 weeks) and evident in the long term (8 weeks) in both grain size and orientation of fibre structures in the tissues analysed (lens and retina). This is the first work devoted to study the effect of FA deficit in the texture of eye tissues using image processing techniques.


Item Type:Article
Additional Information:

Received: 12 July 2018; Revised: 15 October 2018; Accepted: 25 October 2018; First Online: 03 November 2018

Uncontrolled Keywords:Folic acid deficiency; Type IV collagen; Laminin-1; Matlab texture analysis; Retina; Lens
Subjects:Medical sciences > Medicine > Physiology
Medical sciences > Optics > Eyes anatomy
Medical sciences > Optics > Physiological optics
ID Code:51062
Deposited On:05 Feb 2019 12:48
Last Modified:27 Feb 2019 14:33

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