Publication: Desarrollo y validación de nuevas pruebas de campo de inmunidad celular y molecular para la detección de la infección por Leishmania infantum
Loading...
Files
Official URL
Full text at PDC
Publication Date
2019-02-15
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
La leishmaniasis es una de las enfermedades tropicales más desatendidas. El cambio climático, las migraciones masivas, las transformaciones ambientales y las condiciones de inmunodepresión han convertido a esta enfermedad en un serio problema de salud pública. A esto hay que sumarle la falta de medidas de control y de estrategias de eliminación que han ocasionado un aumento de los casos a nivel global, dando lugar a la aparición de nuevos brotes. Esta situación hace que continúe siendo necesario un diagnóstico rápido, fácil y sensible para establecer medidas de control más eficaces. A pesar de ello, las técnicas de diagnóstico actuales para confirmar la leishmaniasis visceral, monitorizar el éxito del tratamiento farmacológico o para detectar recaídas son difíciles de trasladar al campo. Otro desafío importante al que se enfrentan los programas de control es la identificación y manejo de los sujetos con infección asintomática, ya que no se conoce el papel que cumplen dentro de la epidemiología de la enfermedad.El foco principal de esta tesis doctoral ha sido la evaluación de diferentes pruebas de campo para el diagnóstico de los casos tempranos y la detección de la infección asintomática causada por L. infantum y L. donovani, así como el seguimiento tras tratamiento de pacientes con leishmaniasis visceral...
Leishmaniasis is one of the most neglected tropical diseases. Climate change, massive migrations, environmental transformations and immunosuppression conditions have turned this disease into a serious public health problem. The lack of control tools and effective elimination strategies have caused an increase of global cases and new outbreaks. A rapid, easy and sensitive diagnosis is still necessary to establish more effective control measures. Current diagnostic techniques to confirm visceral leishmaniasis, monitor the success of drug treatment or to detect relapses are difficult to transfer to the field. Another important challenge faced by the control programs is the identification and management of subjects with asymptomatic infection, since the role that they play in the epidemiology of the disease is not known.The main focus of this doctoral thesis has been the evaluation of different field tests for the diagnosis of early cases and the detection of asymptomatic infection caused by L. infantum and L. donovani, as well as the follow-up after treatment of patients with leishmaniasis visceral. The six articles included in this thesis are included in a broad objective that pursues the search for field trials and biomarkers of infection/exposure or cure of Leishmania spp. We have evaluated the nucleic acid amplification assay under isothermal conditions (LAMP) together with the Leishmania Loopamp™ detection kit, as a new tool for the diagnosis of active cases, as well as the whole blood stimulation test (WBA) and the analysis of cytokines and chemokines secreted...
Leishmaniasis is one of the most neglected tropical diseases. Climate change, massive migrations, environmental transformations and immunosuppression conditions have turned this disease into a serious public health problem. The lack of control tools and effective elimination strategies have caused an increase of global cases and new outbreaks. A rapid, easy and sensitive diagnosis is still necessary to establish more effective control measures. Current diagnostic techniques to confirm visceral leishmaniasis, monitor the success of drug treatment or to detect relapses are difficult to transfer to the field. Another important challenge faced by the control programs is the identification and management of subjects with asymptomatic infection, since the role that they play in the epidemiology of the disease is not known.The main focus of this doctoral thesis has been the evaluation of different field tests for the diagnosis of early cases and the detection of asymptomatic infection caused by L. infantum and L. donovani, as well as the follow-up after treatment of patients with leishmaniasis visceral. The six articles included in this thesis are included in a broad objective that pursues the search for field trials and biomarkers of infection/exposure or cure of Leishmania spp. We have evaluated the nucleic acid amplification assay under isothermal conditions (LAMP) together with the Leishmania Loopamp™ detection kit, as a new tool for the diagnosis of active cases, as well as the whole blood stimulation test (WBA) and the analysis of cytokines and chemokines secreted...
Description
Tesis de la Universidad Complutense de Madrid, Facultad de Farmacia, Departamento de Parasitología, leída el 27-06-2018