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Osteostatin potentiates the bioactivity of mesoporous glass scaffolds containing Zn2+ ions in human mesenchymal stem cell cultures

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Heras, Clara and Sánchez Salcedo, Sandra and Lozano Borregón, Daniel and Peña, Juan and Esbrit, Pablo and Vallet Regí, María and Salinas Sánchez, Antonio J. (2019) Osteostatin potentiates the bioactivity of mesoporous glass scaffolds containing Zn2+ ions in human mesenchymal stem cell cultures. Acta Biomaterialia . ISSN 1742-7061 (In Press)

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Official URL: https://www.journals.elsevier.com/acta-biomaterialia


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Abstract

There is an urgent need of biosynthetic bone grafts with enhanced osteogenic capacity. In this study, we describe the design of hierarchical meso-macroporous 3D-scaffolds based on mesoporous bioactive glasses (MBGs), enriched with the peptide osteostatin and Zn2+ ions, and their osteogenic effect on human mesenchymal stem cells (hMSCs) as a preclinical strategy in bone regeneration. The MBG compositions investigated were 80%SiO2– 15%CaO–5%P2O5 (in mol-%) Blank (BL), and two analogous glasses containing 4% ZnO (4ZN) and 5% ZnO (5ZN). By using additive fabrication techniques, scaffolds exhibiting hierarchical porosity: mesopores (around 4 nm), macropores (1-600 m) and big channels ( 1000 m), were prepared. These MBG scaffolds with or without osteostatin were evaluated in cell cultures of hMSCs. Zinc promoted hMSCs colonization (both the surface and inside) of MBG scaffolds. Moreover, Zn2+ ions and osteostatin together, but not independently, in the scaffolds were found to induce the osteoblast differentiation genes runt related transcription factor-2 (RUNX2) and alkaline phosphatase (ALP) in hMSCs after 7 d of culture in the absence of an osteogenic differentiation-promoting medium. These results add credence to the combined use of zinc and osteostatin as an effective strategy for bone regeneration applications.


Item Type:Article
Additional Information:

RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
RESEARCHER ID B-5081-2017 (Daniel Lozano Borregón)
ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)
ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)

Uncontrolled Keywords:Mesoporous bioactive glasses, ZnO, Osteostatin, Human mesenchymal cells
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:54704
Deposited On:01 Apr 2019 16:23
Last Modified:02 Apr 2019 10:34

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