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Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics

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Singh, Mandeep and Guzmán Aránguez, Ana Isabel and Hussain, Afzal and Srinivas, Cheerneni S. and Kaur, Indu P. (2019) Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics. Nanomedicine, 14 (4). ISSN 1743-5889

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Official URL: https://doi.org/10.2217/nnm-2018-0278




Abstract

Aim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system.


Item Type:Article
Uncontrolled Keywords:Cellular uptake; Corneal permeability; Cytotoxicity; In vitro release; In vivo ocular toxicity; Minimum inhibitory concentration; Microemulsification; Ocular tissue uptake; Ocular tuberculosis; Stability studies
Subjects:Sciences > Chemistry > Biochemistry
Medical sciences > Optics > Eyes anatomy
ID Code:55110
Deposited On:30 Apr 2019 11:26
Last Modified:30 Apr 2019 11:26

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