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Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes

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Sánchez Tirado, E. and Salvo, C. and Gónzalez Cortés, A and Yáñez Sedeño, Paloma and Langa, F. and Pingarrón Carrazón, José Manuel (2017) Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes. Analytica Chimica Acta, 22 (959). pp. 66-73. ISSN 0003-2670

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/28159106



Abstract

Dual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-PheDWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.


Item Type:Article
Uncontrolled Keywords:Interleukin-1 beta Tumor necrosis factor-alpha Electrochemical immunosensor Multiplex Double-walled carbon nanotubes Saliva
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:55184
Deposited On:07 May 2019 11:10
Last Modified:05 Aug 2019 08:45

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