Universidad Complutense de Madrid
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Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors

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Moreno Guzmán, María and Ojeda, Irene and Villalonga, Reynaldo and González Cortés, Araceli and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2012) Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors. Biosensors and Bioelectronics, 35 . pp. 82-86. ISSN 0956-5663

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Abstract

This work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electrode surfaces. A competitive immunoassay between the antigen and the biotinylated hormone for the binding sites of the immobilized antibody was performed. The electroanalytical response was generated by using alkaline phosphatase-labelled streptavidin and 1-naphtyl phosphate as the enzyme substrate. The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differential pulse voltammetry was employed to monitor the affinity reaction. Under the optimized working conditions, an extremely low detection limit of 18 pg/L was obtained. Cross-reactivity was evaluated against other hormones (cortisol, estradiol, testosterone, progesterone, hGH and prolactin) and the obtained results demonstrated an excellent selectivity. The developed immunosensor was applied to a human serum sample containing a certified amount of ACTH with good results.


Item Type:Article
Uncontrolled Keywords:Adrenocorticotropin hormone; Phenyl boronic acid; Electrochemical immunosensor
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:55218
Deposited On:08 May 2019 11:05
Last Modified:05 Aug 2019 08:49

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