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Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

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Sánchez Tirado, E. and Martínez García, G. and González Cortés, Araceli and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2017) Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine. Biosensors and Bioelectronics, 88 . pp. 9-14. ISSN ISSN: 0956-5663, ESSN: 1873-4235

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/27297187



Abstract

The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.


Item Type:Article
Uncontrolled Keywords:Electrochemical immunosensor; Signal amplification; Transforming growth factor-β1 (TGF-β1); Urine
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:55219
Deposited On:08 May 2019 11:26
Last Modified:05 Aug 2019 08:47

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