Universidad Complutense de Madrid
E-Prints Complutense

Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans.

Impacto

Downloads

Downloads per month over past year

Román González, Elvira and Coman, Ioana V and Prieto, Daniel and Alonso Monge, Rebeca and Pla Alonso, Jesús (2019) Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans. mSphere, 4 (1). ISSN 2379-5042

[img]
Preview
PDF
Creative Commons Attribution.

2MB

Official URL: https://www.asm.org/



Abstract

Clustered regularly interspaced short palindromic repeat (CRISPR) methodology is not only an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and protein interactions. We describe here the implementation of a CRISPR-based system to regulate expression in the clinically important yeast By fusing an allele of Cas9 devoid of nuclease activity to a transcriptional repressor (Nrg1) or activator (Gal4), we were able to show specific repression or activation of the tester gene , encoding the cytosolic catalase. We generated strains where a 1.6-kbp upstream regulatory region of controls the expression of the green fluorescent protein (GFP) and demonstrated the functionality of the constructs by quantitative PCR (qPCR), flow cytometry, and analysis of sensitivity/resistance to hydrogen peroxide. Activation and repression were strongly dependent on the position of the complex in this regulatory region. We also improved transcriptional activation using an RNA scaffolding strategy to allow interaction of inactive variants of Cas9 (dCas9) with the RNA binding protein MCP (monocyte chemoattractant protein) fused to the VP64 activator. The strategy shown here may facilitate the analysis of complex regulatory traits in this fungal pathogen. CRISPR technology is a new and efficient way to edit genomes, but it is also an appealing way to regulate gene expression. We have implemented CRISPR as a gene expression platform in using fusions between a Cas9 inactive enzyme and specific repressors or activators and demonstrated its functionality. This will allow future manipulation of complex virulence pathways in this important fungal pathogen.


Item Type:Article
Uncontrolled Keywords:CRISPR, Candida albicans, Gal4, RNA scaffold, Catalase, Gene activation, Gene repression, Genetic tool
Subjects:Medical sciences > Pharmacy > Cytology
Medical sciences > Pharmacy > Microbiology
ID Code:55654
Deposited On:11 Jun 2019 07:36
Last Modified:17 Jun 2019 10:59

Origin of downloads

Repository Staff Only: item control page