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Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress.

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García, Raúl and Pulido, Verónica and Orellana Muñoz, Sara and Nombela, César and Vázquez de Aldana, Carlos R. and Rodríguez Peña, José Manuel and Arroyo, Javier (2019) Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress. Scientific reports, 9 (1). p. 3186. ISSN 2045-2322

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Abstract

Post-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions.


Item Type:Article
Subjects:Medical sciences > Pharmacy > Cytology
Medical sciences > Pharmacy > Microbiology
ID Code:55659
Deposited On:11 Jun 2019 09:55
Last Modified:17 Jun 2019 10:58

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