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Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.

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Clemente, Luis Felipe and Hernáez, María Luisa and Ramos-Fernández, Antonio and Ligero, Gertrudis and Gil, Concha and Corrales, Fernando José and Marcilla, Miguel (2018) Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord. Journal of proteome research, 17 (12). pp. 4325-4328. ISSN 1535-3907

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Abstract

Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.


Item Type:Article
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:55669
Deposited On:19 Jul 2019 11:04
Last Modified:19 Jul 2019 11:04

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