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Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans

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Vaz, Catarina Oliveira and Reales Calderón, José Antonio and Pitarch, Aida and Vellosillo, Perceval and Trevisan Herraz, Marco and Hernáez Sánchez, Maria Luisa and Monteolliva Díaz, Lucía and Gil García, Concha (2019) Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans. Journal of Proteome Research, 18 (5). pp. 2139-2159. ISSN 1535-3893

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Abstract

Macrophages are involved in the primary human response to Candida albicans. After pathogen recognition, signaling pathways are activated, leading to the production of cytokines, chemokines, and antimicrobial peptides. ATP binding proteins are crucial for this regulation. Here, a quantitative proteomic and phosphoproteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. From a total of 547 nonredundant quantified proteins, 137 were ATP binding proteins and 59 were detected as differentially abundant. From the differentially abundant ATP-binding proteins, 6 were kinases (MAP2K2, SYK, STK3, MAP3K2, NDKA, and SRPK1), most of them involved in signaling pathways. Furthermore, 85 phosphopeptides were quantified. Macrophage proteomic alterations including an increase of protein synthesis with a consistent decrease in proteolysis were observed. Besides, macrophages showed changes in proteins of endosomal trafficking together with mitochondrial proteins, including some involved in the response to oxidative stress. Regarding cell death mechanisms, an increase of antiapoptotic over pro-apoptotic signals is suggested. Furthermore, a high pro-inflammatory response was detected, together with no upregulation of key mi-RNAs involved in the negative feedback of this response. These findings illustrate a strategy to deepen the knowledge of the complex interactions between the host and the clinically important pathogen C. albicans.


Item Type:Article
Additional Information:

Received: January 16, 2019
Published: April 15, 2019

Uncontrolled Keywords:Macrophages, Candida albicans, Proteomics, SILAC, ATP binding proteins
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
Medical sciences > Biology > Microbiology
Medical sciences > Pharmacy > Microbiology
ID Code:55833
Deposited On:19 Jun 2019 11:08
Last Modified:19 Jul 2019 11:05

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