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Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients

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Villanueva Romero, Raúl and Gutiérrez Cañas, Irene and Carrión Caballo, Mar and González Álvaro, Isidoro and Rodríguez Frade, José Miguel and Mellado, Mario and Martínez, Carmen and Gomáriz, Rosa P. and Juarranz Moratilla, Yasmina (2019) Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients. Scientific Reports, 9 (7383). pp. 1-14. ISSN ESSN: 2045-2322

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Official URL: https://www.nature.com/articles/s41598-019-43717-2



Abstract

Vasoactive Intestinal Peptide (VIP) is an important immunomodulator of CD4+ cells in normal and pathological conditions, which exerts its anti-infammatory and immunomodulatory actions through VPAC receptors, VPAC1 and VPAC2. Only a decrease in the expression of VPAC1 mRNA on Th cells upon activation has been reported. Thus, the deepening in the knowledge of the behavior of these receptors may contribute to the design of new therapies based on their activation and/or blockade. In this study, we describe the expression pattern, cellular location and functional role of VIP receptors during the activation of human Th cells in healthy conditions and in early arthritis (EA). The protein expression pattern of VPAC1 did not change with the activation of Th lymphocytes, whereas VPAC2 was up-regulated. In resting cells, VPAC1 was located on the plasma membrane and nucleus, whereas it only appeared in the nucleus in activated cells. VPAC2 was always found in plasma membrane location. VIP receptors signaled through a PKA-dependent pathway in both conditions, and also by a PKAindependent pathway in activated cells. Both receptors exhibit a potent immunomodulatory capacity by controlling the pathogenic profle and the activation markers of Th cells. These results highlight a novel translational view in infammatory/autoimmune diseases.


Item Type:Article
Uncontrolled Keywords:Th lymphocytes; Vasoactive Intestinal Peptide; Arthritis
Subjects:Medical sciences > Medicine > Immunology
Medical sciences > Medicine > Rheumatology
Medical sciences > Biology > Biochemistry
ID Code:56620
Deposited On:30 Jul 2019 10:27
Last Modified:30 Jul 2019 11:05

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