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Rapid micromotor-based naked-eye immunoassay

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Esteban Fernandez de Avila, Berta and Zhao, Mingjiao and Campuzano, Susana and Ricci, Francesco and Pingarrón Carrazón, José Manuel and Mascini, Marcelo and Wang, Joseph (2017) Rapid micromotor-based naked-eye immunoassay. Talanta, 167 . pp. 651-657. ISSN 0039-9140

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Official URL: http://www.sciencedirect.com/science/article/pii/S0039914017302825



Abstract

A dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micromotors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H2O2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables “on the move” specific detection of the target cortisol down to 0.1 μg mL−1 in just 2 min, using ultrasmall (50 µL) sample volumes.


Item Type:Article
Uncontrolled Keywords:Cortisol, Real-time sensing, Micromotor, Antibody, Naked-eye detection
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:56685
Deposited On:02 Aug 2019 10:19
Last Modified:05 Aug 2019 08:48

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