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Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction

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Álvarez-Campos, Patricia and Kenny, Nathan J. and Verdes, Aida and Fernández, Rosa and Novo Rodríguez, Marta and Giribet, Gonzalo and Riesgo, Ana (2019) Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction. Genome Biology and Evolution, 11 (1). pp. 295-318. ISSN 1759-6653

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Official URL: https://academic.oup.com/gbe/article/11/1/295/5238077



Abstract

Stolonizationinsyllidannelids is auniquemodeof reproductionamonganimals.Duringthebreedingseason, a structure resemblingthe adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal.When stolons mature, they detachfromthe adult andgametes are released intothewater column. Theprocess is synchronizedwithineachspecies, and it hasbeen reportedtobeunder environmental andendogenous control,probably via endocrine regulation. Tofurtherunderstandreproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllismagdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. Inaddition,our findings suggest that severalneurohormones, suchasmethyl farnesoate,dopamine, andserotonin,might trigger stolon formation, the correct maturation of gametes and the detachment of stolonswhen gametogenesis ends. The process seems tobe under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of howsome hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization.

Resumen (otros idiomas)

Los sílidos son un grupo de gusanos marinos sorprendentes por su modo de reproducción: desarrollan pequeños “clones” de sí mismos al final de su cuerpo (estolones), que sólo contienen óvulos o espermatozoides. Cuando los estolones están completamente maduros, se desprenden del adulto para nadar hasta la superficie donde liberarán los gametos de forma sincronizada (siguiendo el ciclo lunar). Hasta ahora se sabía que el proceso estaba controlado por hormonas, pero gracias a nuestro trabajo de expresión génica durante la reproducción de una especie del Pacífico, hemos conseguido averiguar un poco más sobre los genes que participan en el proceso. La intervención de dos de las hormonas más conocidas por regular actividades importantes del cerebro de numerosos animales (Dopamina y Serotonina), así como una tercera similar a la hormona juvenil de los insectos (que les permite su metamorfosis a adultos), son algunos de los resultados más llamativos de nuestro trabajo

Item Type:Article
Uncontrolled Keywords:Transcriptomics, stolonizing syllids, reproduction, hormonal control
Subjects:Medical sciences > Biology > Marine biology
Medical sciences > Biology > Invertebrates
ID Code:56772
Deposited On:20 Aug 2019 18:02
Last Modified:26 Aug 2019 07:36

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