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Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

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Orea-Soufi, Alba and Dávila, David and Salazar Roa, María and Lorente Pérez, María del Mar and Velasco Díez, Guillermo (2019) Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity. In FOXO Transcription Factors: Methods and Protocols. Methods in Molecular Biology (1890). Springer, Madrid, pp. 51-59. ISBN 978-1-4939-8900-3

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Official URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-8900-3_5



Abstract

Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here, we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.


Item Type:Book Section
Uncontrolled Keywords:FOXO phosphorylation, Western blot, Immunofluorescence, cell fractioning
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:56792
Deposited On:22 Aug 2019 17:24
Last Modified:26 Aug 2019 08:04

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