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Significance of Aspergillus nigeraggregate species as contaminants of food products in Spain regarding their occurrence and their ability to produce mycotoxins

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Gil-Serna, Jessica and García-Díaz, Marta and Vázquez, Covadonga and González Jaén, María Teresa and Patiño Álvarez, Belén (2019) Significance of Aspergillus nigeraggregate species as contaminants of food products in Spain regarding their occurrence and their ability to produce mycotoxins. Food Microbiology, 82 . pp. 240-248. ISSN 1095-9998

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Official URL: https://www.sciencedirect.com/science/article/pii/S0740002018308360?via%3Dihub



Abstract

The Aspergillus niger aggregate contains 15 morphologically indistinguishable species which presence is related to ochratoxin A (OTA) and fumonisin B2 (FB2) contamination of foodstuffs. The taxonomy of this group was recently reevaluated and there is a need of new studies regarding the risk that these species might pose to food security. 258 isolates of A. niger aggregate obtained from a variety of products from Spain were classified by molecular methods being A. tubingensis the most frequently occurring (67.5%) followed by A. welwitschiae (19.4%) and A. niger (11.7%). Their potential ability to produce mycotoxins was evaluated by PCR protocols which allow a rapid detection of OTA and FB2 biosynthetic genes in their genomes. OTA production is not widespread in A. niger aggregate since only 17% of A. niger and 6% of A. welwitschiae isolates presented the complete biosynthetic cluster whereas the lack of the cluster was confirmed in all A. tubingensis isolates. On the other hand, A. niger and A. welwitschiae seem to be important FB2 producers with 97% and 29% of the isolates, respectively, presenting the complete cluster. The genes involved in OTA and FB2 were overexpressed in producing isolates and their expression was related to mycotoxin synthesis.

Resumen (otros idiomas)

El grupo de especies del agregado de Aspergillus niger incluye importantes hongos contaminantes de alimentos que suponen un grave riesgo para la seguridad alimentaria debido a su capacidad de producir micotoxinas como la ocratoxina A o fumonisina B2, dos posibles carcinógenos humanos. En este trabajo, hemos estudiado en profundidad cual es la incidencia de estas especies en los productos alimentarios más frecuentemente contaminados por micotoxinas en España y su potencial capacidad para producir micotoxinas. La especie más frecuentemente detectada fue A. tubingensis con más de un 65% de los aislamientos en prácticamente todos los alimentos analizados. Sin embargo, ninguna de las cepas analizadas es potencial productora de ninguna de las toxinas ya que no presenta los genes de producción en su genoma. Por otro lado, A. niger y A. welwitschiae fueron aislados menos frecuentemente pero la mayoría sí son potenciales productores de toxinas y, por tanto, su presencia en alimentos debe ser tenida en cuenta para establecer medidas de control efectivas.

Item Type:Article
Uncontrolled Keywords:Ochratoxin A, Fumonisins, Food safety, Section Nigri
Subjects:Medical sciences > Biology > Genetics
Medical sciences > Biology > Microbiology
ID Code:57051
Deposited On:20 Sep 2019 15:26
Last Modified:23 Apr 2020 11:31

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