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Xanthohumol exerts protective effects in liver alterations associated with aging

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Fernández-García, Cristina and Rancan, Lisa and Paredes Royano, Sergio Damián and Montero, César and Fuente del Rey, Mónica de la and Vara Ameigeiras, Elena and Tresguerres, Jesús A. (2019) Xanthohumol exerts protective effects in liver alterations associated with aging. European Journal of Nutrition, 58 (2). pp. 653-663. ISSN 1436-6215

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Official URL: https://link.springer.com/article/10.1007%2Fs00394-018-1657-6



Abstract

Background and aims
Aging is associated with a deregulation of biological systems that lead to an increase in oxidative stress, inflammation, and apoptosis, among other effects. Xanthohumol is the main preylated chalcone present in hops (Humulus lupulus L.) whose antioxidant, anti-inflammatory and chemopreventive properties have been shown in recent years. In the present study, the possible protective effects of xanthohumol on liver alterations associated with aging were evaluated.

Methods
Male young and old senescence-accelerated prone mice (SAMP8), aged 2 and 10 months, respectively, were divided into four groups: non-treated young, non-treated old, old treated with 1 mg/kg/day xanthohumol, and old treated with 5 mg/kg/day xanthohumol. Male senescence-accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed and livers were collected. mRNA (AIF, BAD, BAX, Bcl-2, eNOS, HO-1, IL-1β, NF-κB2, PCNA, sirtuin 1 and TNF-α) and protein expressions (BAD, BAX, AIF, caspase-3, Blc-2, eNOS, iNOS, TNF-α, IL1β, NF-κB2, and IL10) were measured by RT-PCR and Western blotting, respectively. Mean values were analyzed using ANOVA.

Results
A significant increase in mRNA and protein levels of oxidative stress, pro-inflammatory and proliferative markers, as well as pro-apoptotic parameters was shown in old non-treated SAMP8 mice compared to the young SAMP8 group and SAMR1 mice. In general, age-related oxidative stress, inflammation and apoptosis were significantly decreased (p < 0.05) after XN treatment. In most cases, this effect was dose-dependent.

Conclusions
XN was shown to modulate inflammation, apoptosis, and oxidative stress in aged livers, exerting a protective effect in hepatic alterations.


Item Type:Article
Uncontrolled Keywords:Liver, Aging, Inflammation, Senescence-accelerated mouse, Xanthohumol
Subjects:Medical sciences > Medicine > Gastroenterology and Hepatology
Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:57072
Deposited On:23 Sep 2019 14:24
Last Modified:23 Sep 2019 14:24

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