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Comparison of cell mechanical measurements provided by Atomic ForceMicroscopy (AFM) and Micropipette Aspiration (MPA)

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Daza, Rafael and González Bermúdez, Blanca and Cruces, Julia and Fuente del Rey, Mónica de la and Plaza, Gustavo R. and Arroyo Hernández, María and Elices, Manuel and Pérez Rigueiro, José and Guinea, Gustavo V. (2019) Comparison of cell mechanical measurements provided by Atomic ForceMicroscopy (AFM) and Micropipette Aspiration (MPA). Journal of the Mechanical Behavior of Biomedical Materials, 95 . pp. 103-115. ISSN 1878-0180

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Official URL: https://www.sciencedirect.com/science/article/pii/S1751616118317934?via%3Dihub



Abstract

A comparative analysis of T-lymphocyte mechanical data obtained from Micropipette Aspiration (MPA) andAtomic Force Microscopy (AFM) is presented. Results obtained byfitting the experimental data to simple Hertzand Theret models led to non-Gaussian distributions and significantly different values of the elastic moduliobtained by both techniques. The use of more refined models, taking into account thefinite size of cells (sim-plified double contact and Zhou models) reduces the differences in the values calculated for the elastic moduli.Several possible sources for the discrepancy between the techniques are considered. The analysis suggests thatthe local nature of AFM measurements compared with the more general character of MPA measurementsprobably contributed to the differences observed.


Item Type:Article
Uncontrolled Keywords:MechanobiologyT cell, Elastic modulus, Classical models, Finite size effect
Subjects:Medical sciences > Biology > Cytology
ID Code:57424
Deposited On:21 Oct 2019 12:13
Last Modified:21 Oct 2019 12:13

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