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Prokaryotic and viral community of the sulfate‐rich crust from Peñahueca ephemeral lake, an astrobiology analogue

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Martin Cuadrado, Ana Belen and Senel, Ece and Martínez García, Manuel and Cifuentes, Ana and Santos, Fernando and Almansa, Cristina and Moreno Paz, Mercedes and Blanco, Yolanda and García Villadangos, Miriam and García del Cura, M. Ángeles and Sanz Montero, M. Esther and Rodríguez Aranda, Juan Pablo (2019) Prokaryotic and viral community of the sulfate‐rich crust from Peñahueca ephemeral lake, an astrobiology analogue. Environmental Microbiology, 21 (10). pp. 3577-3600. ISSN 1462-2912, ESSN: 1462-2920

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Official URL: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.14680



Abstract

Peñahueca is an athalassohaline hypersaline inland ephemeral lake originated under semiarid conditions in the central Iberian Peninsula (Spain). Its chemical composition makes it extreme for microbial life as well as a terrestrial analogue of other planetary environments. To investigate the persistence of microbial life associated with sulfate‐rich crusts, we applied cultivation‐independent methods (optical and electron microscopy, 16S rRNA gene profiling and metagenomics) to describe the prokaryotic community and its associated viruses. The diversity for Bacteria was very low and was vastly dominated by endospore formers related to Pontibacillus marinus of the Firmicutes phylum. The archaeal assemblage was more diverse and included taxa related to those normally found in hypersaline environments. Several ‘metagenome assembled genomes’ were recovered, corresponding to new species of Pontibacillus, several species from the Halobacteria and one new member of the Nanohaloarchaeota. The viral assemblage, although composed of the morphotypes typical of high salt systems, showed little similarity to previously isolated/reconstructed halophages. Several putative prophages of Pontibacillus and haloarchaeal hosts were identified. Remarkably, the Peñahueca sulfate‐rich metagenome contained CRISPR‐associated proteins and repetitions which were over 10‐fold higher than in most hypersaline systems analysed so far.


Item Type:Article
Subjects:Sciences > Geology > Geochemistry
Sciences > Geology > Petrology
Medical sciences > Biology > Microbiology
ID Code:57504
Deposited On:28 Oct 2019 19:31
Last Modified:29 Oct 2019 10:13

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