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Per- and polyfluoroalkyl substances (PFASs) modify lung surfactant function and pro-inflammatory responses in human bronchial epithelial cells

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Sørli, Jorid B. and Låg, Marit and Ekerenb, Leni and Pérez-Gil, Jesús and Haug, Line S. H and Da Silva, Emilie and Matrod, Muhammad N. and Gützkow, Kristine B. and Lindeman, Birgitte (2020) Per- and polyfluoroalkyl substances (PFASs) modify lung surfactant function and pro-inflammatory responses in human bronchial epithelial cells. Toxicology in Vitro, 62 (104656). pp. 1-9. ISSN 0887-2333, ESSN: 1879-3177

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Official URL: https://www.sciencedirect.com/science/article/pii/S0887233319304795



Abstract

The toxicity of some per- and polyfluoroalkyl substances (PFASs), such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) has been studied thoroughly, showing that systemic PFASs targets the lungs. However, regulators lack data to assess the impact of other PFASs on the lungs and alternative methods to test substances for lung toxicity are needed. We combined two in vitro models to assess toxicity to the respiratory system; i) a lung surfactant (LS) function assay to assess the acute inhalation toxicity potential, and ii) a cell model with human bronchial epithelial cells to study pro-inflammatory potential and modulation of inflammatory responses. We tested salts of four PFASs: perfluorobutane sulfonate (PFBS), perfluorohexane sulfonate (PFHxS), PFOS, and PFOA as well as the fluorotelomer 8:2 FTOH. The results show that PFHxS, PFOA and PFOS can inhibit LS function. High PFOS concentrations induced a pro-inflammatory response, measured as increased IL-1α/β release. Moderate concentrations of PFOS suppressed release of the chemokines CXCL8 and CXCL10, whereas both PFOS and PFOA stimulated the release of the pro-inflammatory cytokine IL-1β in immune stimulated human bronchial epithelial cells. These findings support the concern that some PFASs may increase the risk of acute lung toxicity and of airway infections.


Item Type:Article
Uncontrolled Keywords:Acute inhalation toxicity; Lung surfactant; Constrained drop surfactometer; Pro-inflammatory responses; Bronchial epithelial cells; Alternative methods
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:57538
Deposited On:31 Oct 2019 11:07
Last Modified:31 Oct 2019 11:11

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