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Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep.

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Casarrubios, L. and Gómez-Cerezo, Natividad and Sánchez Salcedo, Sandra and Feito Castellano, María José and Serrano, M.C. and Saiz-Pardo, M. and Ortega Menor, Lorena and De Pablo, D. and Díaz-Güemes, I. and Fernández-Tomé, E. and Enciso, S and Portolés Pérez, María Teresa and Sanchez-Margallo, F.M and Arcos Navarrete, Daniel and Vallet Regí, María and Sanchez-Margallo, F. M. (2019) Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep. Acta Biomaterialia . ISSN 1742-7061 (In Press)

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Official URL: https://www.journals.elsevier.com/acta-biomaterialia


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Abstract

Silicon-substituted hydroxyapatite (SiHA) macroporous scaffolds have been prepared by robocasting. In order to optimize their bone regeneration properties, we have manufactured these scaffolds presenting different microstructures: nanocrystalline and crystalline. Moreover, their surfaces have been decorated with vascular endothelial growth factor (VEGF) to evaluate the potential coupling between vascularization and bone regeneration. In vitro cell culture tests evidence that nanocrystalline SiHA hinders pre-osteblast proliferation, whereas the presence of VEGF enhances the biological functions of both endothelial cells and pre-osteoblasts. The bone regeneration capability has been evaluated using an osteoporotic sheep model. In vivo observations strongly correlate with in vitro cell culture tests. Those scaffolds made of nanocrystalline SiHA were colonized by fibrous tissue, promoted inflammatory response and forested osteoclast recruitment. These observations discard nanocystalline SiHA as a suitable material for bone regeneration purposes. On the contrary, those scaffolds made of crystalline SiHA and decorated with VEGF exhibited bone regeneration properties, with high ossification degree, thicker trabeculae and higher presence of osteoblasts and blood vessels. Considering these results, macroporous scaffolds made of SiHA and decorated with VEGF are suitable bone grafts for regeneration purposes, even in adverse pathological scenarios such as osteoporosis.


Item Type:Article
Additional Information:

RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)

Uncontrolled Keywords:Silicon substituted hydroxyapatite, Macroporous scaffold, VEGF, Osteoporosis, In vivo test
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:57824
Deposited On:26 Nov 2019 12:01
Last Modified:26 Nov 2019 13:03

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