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Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production

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Guevara, Govinda and Olortegui Flores, Yamileth and Fernández de las Heras, Laura and Perera González, Julián and Navarro LLorens, Juana María (2019) Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production. Plos One, 14 (7). pp. 1-16. ISSN 1932-6203

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Official URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220492



Abstract

Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.


Item Type:Article
Uncontrolled Keywords:Rhodococcus Chol-4; Testosterone
Subjects:Medical sciences > Biology > Biochemistry
Medical sciences > Biology > Microbiology
ID Code:57927
Deposited On:27 Nov 2019 09:28
Last Modified:27 Nov 2019 09:43

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