Publication: Derivados de bis(benzimidazol) como nuevo vector para el estudio de la interacción de carbohidratos con el surco menor del ADN
Loading...
Official URL
Full text at PDC
Publication Date
2019-12-11
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
El objetivo general de la presente tesis es poder cuantificar la contribución a la interacción de los carbohidratos con el interior del surco menor del ADN. Para ello, el primer objetivo es el diseño de un nuevo tipo de vector que acerque al carbohidrato hasta el surco menor del ADN. En este contexto, los ligandos de la familia Hoechst se ajustan a nuestro objetivo debido a que su interacción con el surco menor del ADN es bien conocida, presentando una elevada afinidad de secuencias AT y, además, el proceso de agregación que presentan ha sido estudiado y cuantificado por técnicas de microcalorimetría. Por otro lado, los trabajos de Nielsen y Jacobsen presentan un análogo de Hoechst con un grupo ácido en un extremo, lo que posibilita la formación de enlaces amida (Figura 1). Dicha estructura es ideal para nuestros objetivos...
The main objective of this PhD thesis is to quantify the carbohydrate contribution to the interaction with the DNA minor groove. To achieve this, the first objective is designing a new vector type molecule able to carry the carbohydrates into the inner region of the DNA minor groove. On this contest, Hoechst ligands fit to our objective due to the fact that their interaction with the DNA minor groove is well known, showing a high AT sequences affinity and, additionally, their aggregation process has been studied and quantify by microcalorimetry. On the other hand, Nielsen and Jacobsen reported a Hoechst analogue whose structure contains an acid group at a terminal position, allowing the formation of an amide bond (Figure 1) between the vector and the sugar. This structure is excellent for our objectives...
The main objective of this PhD thesis is to quantify the carbohydrate contribution to the interaction with the DNA minor groove. To achieve this, the first objective is designing a new vector type molecule able to carry the carbohydrates into the inner region of the DNA minor groove. On this contest, Hoechst ligands fit to our objective due to the fact that their interaction with the DNA minor groove is well known, showing a high AT sequences affinity and, additionally, their aggregation process has been studied and quantify by microcalorimetry. On the other hand, Nielsen and Jacobsen reported a Hoechst analogue whose structure contains an acid group at a terminal position, allowing the formation of an amide bond (Figure 1) between the vector and the sugar. This structure is excellent for our objectives...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Químicas, leída el 29/10/2019