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Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes

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Rodrigo Martín-Romo, José Augusto and Soto Rueda, Juan Manuel and Alieva, Tatiana Krasheninnikova (2019) Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes. In Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV. Proceedings of SPIE (11060). Society of Photo-Optical Instrumentation Engineers (SPIE), Belliningham, Washington. ISBN 978-1-5106-2800-7

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Official URL: http://dx.doi.org/10.1117/12.2525840




Abstract

Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.


Item Type:Book Section
Additional Information:

© 2019 Society of Photo-Optical Instrumentation Engineers (SPIE).
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (4º. 2019. Munich)
ISSN 0277-786X.
The funding of the projects TEC2014-57394-P (Ministerio de Economía y Competitividad) and PR75/18-21567 (Santander-UCM) is acknowledged.

Uncontrolled Keywords:Partially coherent-Light; Reconstruction; Algorithms
Subjects:Sciences > Physics > Optics
ID Code:58614
Deposited On:21 Jan 2020 17:11
Last Modified:21 Jan 2020 17:11

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