Publication: Análisis transcriptómico comparativo de "Porphyromonas gingivalis" ATCC 33277 en diferentes estados fenotípicos
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2020-02-10
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Universidad Complutense de Madrid
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Abstract
El objetivo general de esta tesis es determinar y comparar los cambios de expresión génica global de P. gingivalis ATCC 33277, mediante análisis transcriptómico con el uso de microarrays , en diferentes condiciones in vitro. Los objetivos específicos son: primero, identificar genes regulados diferencialmente por P. gingivalis ATCC 33277 en un estado planctónico puro y en uno en presencia de un biofilm monoespecie. Segundo, estudiar los cambios de expresión génica global de P. gingivalis ATCC 33277, en estado planctónico y en un modelo in vitro de biofilm estático monoespecie. Y tercero,estudiar y comparar los cambios de expresión génica global de P. ingivalis ATCC 33277 en estado planctónico y asociado a un modelo estático in vitro de biofilm multiespecie, con el fin de ampliar el conocimiento de las bases moleculares de la formación del biofilm y los cambios que se producen por la interacción de este periodontopatógeno con otras especies...
The general objective of this thesis is to determine and compare the global gene expression changes of P. ingivalis ATCC 33277, by means of transcriptomic analysis with the use of microarrays, under different conditions in vitro. The specific objectives are: First: To identify genes differentially regulated by P. Gingivalis ATCC 33277 in a pure planktonic state and in one in the presence of a monospecies biofilm. Second: to study the global gene expression changes of P. Gingivalis ATCC 33277, in the planktonic state and in an in vitro model of static monospecies biofilm. And third : to study and compare the global gene expression changes of P. Gingivalis ATCC 33277 in a planktonic state and associated with a static in vitro model of multispecies biofilm, in order to broaden knowledge of the molecular basis of biofilm formation and the changes that are produced by the interaction of this periodontopathogen with other species...
The general objective of this thesis is to determine and compare the global gene expression changes of P. ingivalis ATCC 33277, by means of transcriptomic analysis with the use of microarrays, under different conditions in vitro. The specific objectives are: First: To identify genes differentially regulated by P. Gingivalis ATCC 33277 in a pure planktonic state and in one in the presence of a monospecies biofilm. Second: to study the global gene expression changes of P. Gingivalis ATCC 33277, in the planktonic state and in an in vitro model of static monospecies biofilm. And third : to study and compare the global gene expression changes of P. Gingivalis ATCC 33277 in a planktonic state and associated with a static in vitro model of multispecies biofilm, in order to broaden knowledge of the molecular basis of biofilm formation and the changes that are produced by the interaction of this periodontopathogen with other species...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Odontología, Departamento de Especialidades Clínicas Odontológicas, leída el 30-09-2019