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Microglial Activation in the Retina of a Triple-Transgenic Alzheimer’s Disease Mouse Model (3xTg-AD)

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García Martín, Elena Salobrar and Rodríguez Neves, Ana C. and Ramírez Sebastián, Ana Isabel and Hoz Montañana, María Rosa de and Fernández Albarral, José Antonio and López Cuenca, Inés and Ramirez Sebastian, Jose Manuel and Ambrósio, Antonio Francisco and Salazar Corral, Juan José (2020) Microglial Activation in the Retina of a Triple-Transgenic Alzheimer’s Disease Mouse Model (3xTg-AD). International Journal of Molecular Sciences, 21 (3). p. 816. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms21030816




Abstract

Alzheimer’s disease (AD) is the most common type of dementia in the world. The main biomarkers associated with AD are protein amyloid-β (Aβ) plaques and protein tau neurofibrillary tangles, which are responsible for brain neuroinflammation mediated by microglial cells. Increasing evidence has shown that the retina can also be affected in AD, presenting some molecular and cellular changes in the brain, such as microglia activation. However, there are only a few studies assessing such changes in the retinal microglia in animal models of AD. These studies use retinal sections, which have some limitations. In this study, we performed, for the first time in a triple-transgenic AD mouse model (3xTg-AD), a quantitative morphometric analysis of microglia activation (using the anti-Iba-1 antibody) in retinal whole-mounts, allowing visualization of the entire microglial cell, as well as its localization along the extension of the retina in different layers. Compared to age-matched animals, the retina of 3xTg-AD mice presents a higher number of microglial cells and a thicker microglial cell body area. Moreover, the microglia migrate, reorient, and retract their processes, changing their localization from a parallel to a perpendicular position relative to the retinal surface. These findings demonstrate clear microglia remodeling in the retina of 3xTg-AD mice.


Item Type:Article
Additional Information:

This article belongs to the Special Issue Molecular Mechanism of Alzheimer's Disease II
Received: 19 December 2019 / Revised: 24 January 2020 / Accepted: 25 January 2020 / Published: 27 January 2020

Uncontrolled Keywords:Alzheimer’s disease; Retina; Neuroinflammation; Microglia; Triple transgenic Alzheimer’s disease mouse model; 3xTg-AD; Morphometric analysis
Subjects:Medical sciences > Medicine > Neurosciences
Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
ID Code:58894
Deposited On:28 Feb 2020 13:09
Last Modified:02 Mar 2020 09:51

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