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Bacteria as Nanoparticles Carrier for Enhancing Penetration in a Tumoral Matrix Model

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Moreno Zafra, Víctor Manuel and Alvarez Corchado, Elena and Izquierdo-Barba, Isabel and Baeza, Alejandro and Serrano López, Juana and Vallet Regí, María (2020) Bacteria as Nanoparticles Carrier for Enhancing Penetration in a Tumoral Matrix Model. Advanced Materials Interfaces . ISSN 2196-7350 (In Press)

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Official URL: https://onlinelibrary.wiley.com/journal/21967350




Abstract

One of the major concerns in the application of nanocarriers in oncology is their scarce penetration capacity in tumoral tissues, which drastically compromises the effectivity. Living organisms as cells and bacteria present the capacity to navigate autonomously following chemical gradients being
able to penetrate deeply into dense tissues. In the recent years, the possibility to employ these organisms for the transportation of therapeutic agents and nanocarriers attached on their membrane or engulfed in their inner space have received huge attention. Herein, based on this principle, a new approach to deliver drug loaded nanoparticles achieving high penetration in tumoral matrices is presented. In this case, Escherichia coli (E. coli) bacteria wall is decorated with azide groups, whereas alkyne-strained groups are incorporated on the surface of mesoporous silica nanoparticles loaded with a potent cytotoxic compound, doxorubicin. Both functional groups form stable triazole bonds by click-type reaction allowing the covalent grafting of nanoparticles on living bacteria. Thus, the motility and penetration capacity of bacteria, which carried nanoparticles are evaluated in a 3D tumoral matrix model composed by a dense collagen extracellular matrix with HT1080 human fibrosarcome cells embedded. The results confirmed that bacteria are able to transport the nanoparticles crossing a thick collagen layer being able to
destroy almost 80% of the tumoral cells located underneath. These findings envision a powerful strategy in nanomedicine applied for cancer treatment by Q4 allowing a homogeneous distribution of therapeutic agents in the malignancy.


Item Type:Article
Additional Information:

RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)
ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)
RESEARCHER ID K-8193-2014 (Alejandro Baeza)
ORCID 0000-0002-2026-6266 (Alejandro Baeza)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Bacteria motors, Mesoporous silica nanoparticles, Nanocarriers tumor penetration, Nanomedicine.
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:59860
Deposited On:11 May 2020 08:32
Last Modified:13 May 2020 08:24

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