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Rhodamine-based sensor for real-time imaging of mitochondrial ATP in living fibroblasts

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de la Fuente Herreruela, Diego and Gónzalez Charro, Vicente and Almendro Vedia, Víctor G. and Morán, María and Martín, Miguel Ángel and Lillo, M. Pilar and Natale, Paolo and López-Montero, Iván (2017) Rhodamine-based sensor for real-time imaging of mitochondrial ATP in living fibroblasts. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1858 (12). pp. 999-1006. ISSN 0005-2728

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Official URL: https://doi.org/10.1016/j.bbabio.2017.09.004



Abstract

Mitochondria are essential for the production and maintenance of ATP in the eukaryotic cell. To image and monitor intracellular ATP level without cell breakage, biological and chemical sensors were developed in the last years. Here, we have internalized a rhodamine-based sensor RSL+ into living cells and monitored the mitochondrial ATP levels in cultured mouse embryonic fibroblasts. To evaluate the robustness of the sensor we imaged the changes of the mitochondrial ATP levels under non-physiological conditions upon incubation with FCCP, oligomycin, azide, deoxyglucose or phosphoenolpyruvate; all compounds that interfere with ATP homeostasis of the cell. The ATP sensor allowed us to determine the mitochondrial ATP levels in human skin fibroblasts where we observe a similar amount of ATP compared to mouse embryonic fibroblasts. We propose the RSL+ to be a valuable tool for the assessment of mitochondrial dysfunction in human cells derived from mitochondrial OXPHOS patients and for basic studies on bioenergetics metabolism.


Item Type:Article
Additional Information:

The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)

Uncontrolled Keywords:ATP sensor; Mitochondrial diseases; ATP mapping
Subjects:Sciences > Chemistry > Chemistry, Physical and theoretical
Medical sciences > Biology > Biotechnology
ID Code:60118
Deposited On:22 Apr 2020 18:35
Last Modified:23 Apr 2020 07:50

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