Universidad Complutense de Madrid
E-Prints Complutense

Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery

Impacto

Downloads

Downloads per month over past year

de la Fuente Herreruela, Diego and Monnappa, Ajay K. and Muñoz Úbeda, Mónica and Morallón Piña, Aarón and Enciso Rodríguez, Eduardo and Sánchez Martín, Luis and Giusti, Fabrice and Natale, Paolo and López-Montero, Iván (2019) Lipid–peptide bioconjugation through pyridyl disulfide reaction chemistry and its application in cell targeting and drug delivery. Journal of Nanobiotechnology, 17 (1). ISSN 1477-3155

[img] PDF
Creative Commons Attribution.

1MB

Official URL: https://doi.org/10.1186/s12951-019-0509-8



Abstract

Background: The design of efcient drug delivery vectors requires versatile formulations able to simultaneously direct a multitude of molecular targets and to bypass the endosomal recycling pathway of cells. Liposomal-based vectors need the decoration of the lipid surface with specifc peptides to fulfll the functional requirements. The unspecifc binding of peptides to the lipid surface is often accompanied with uncontrolled formulations and thus preventing the molecular mechanisms of a successful therapy.

Results: We present a simple synthesis pathway to anchor cysteine-terminal peptides to thiol-reactive lipids for ade quate and quantitative liposomal formulations. As a proof of concept, we have synthesized two diferent lipopeptides based on (a) the truncated Fibroblast Growth Factor (tbFGF) for cell targeting and (b) the pH sensitive and fusogenic GALA peptide for endosomal scape.

Conclusions: The incorporation of these two lipopeptides in the liposomal formulation improves the fbroblast cell targeting and promotes the direct delivery of cargo molecules to the cytoplasm of the cell. Keywords: Smart liposomes, Disulfde bonds, Targeting peptide, GALA, Endosomal escape.


Item Type:Article
Additional Information:

The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)

Uncontrolled Keywords:Smart liposomes, Disulfde bonds, Targeting peptide, GALA, Endosomal escape
Subjects:Sciences > Chemistry
Sciences > Chemistry > Chemistry, Physical and theoretical
Medical sciences > Biology > Biotechnology
ID Code:60124
Deposited On:23 Apr 2020 07:34
Last Modified:23 Apr 2020 07:35

Origin of downloads

Repository Staff Only: item control page