Universidad Complutense de Madrid
E-Prints Complutense

Recent ddvances in practical methods for liver cell biology: a short overview

Impacto

Downloads

Downloads per month over past year

Torres, Sandra and Abdullah, Zeinab and Brol, Maximilian J. and Hellerbrand, Claus and Fernández, Mercedes and Fiorotto, Romina and Klein, Sabine and Königshofer, Philipp and Liedtke, Christian and Lotersztajn, Sophie and Nevzorova, Yulia A. and Schierwagen, Robert and Reiberger, Thomas and Uschner, Frank Erhard and Tacke, Frank and Weiskirchen, Ralf and Trebicka, Jonel (2020) Recent ddvances in practical methods for liver cell biology: a short overview. International Journal of Molecular Sciences, 21 (6). pp. 1-19. ISSN 1661-6596, ESSN: 1422-0067

[img]
Preview
PDF
Creative Commons Attribution.

1MB

Official URL: https://www.mdpi.com/1422-0067/21/6/2027



Abstract

Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.


Item Type:Article
Uncontrolled Keywords:Hepatic stellate cells; Hepatocellular cancer; Fibrogenesis; Steatosis; In vitro models
Subjects:Medical sciences > Medicine > Gastroenterology and Hepatology
Medical sciences > Biology > Cytology
ID Code:60724
Deposited On:02 Jun 2020 08:31
Last Modified:02 Jun 2020 17:16

Origin of downloads

Repository Staff Only: item control page