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Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids

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Oeo-Santos, Carmen and López Rodríguez, Juan C. and García-Mouton, Cristina and San Segundo-Acosta, Pablo and Jurado, Aurora and Moreno-Aguilar, Carmen and García-Álvarez, Begoña and Pérez Gil, Jesús and Villalba, Mayte and Barderas Manchado, Rodrigo and Cruz Rodríguez, Antonio (2020) Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1862 (6). pp. 1-9. ISSN 0005-2736

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Official URL: https://www.sciencedirect.com/science/article/abs/pii/S0005273620300833



Abstract

Ole e 7 allergen from Olea europaea pollen possesses a major clinical relevance because it produces severe symptoms, such as anaphylaxis, in allergic patients exposed to high olive pollen counts. Ole e 7 is a non-specific lipid transfer protein (nsLTP) characterized by the presence of a tunnel-like hydrophobic cavity, which may be suitable for hosting and, thus, transporting lipids -as it has been described for other nsLTPs-. The identification of the primary amino acid sequence of Ole e 7, and its production as a recombinant allergen, allowed characterizing its lipid-binding properties and its effect at air-liquid interfaces. Fluorescence and interferometry experiments were performed using different phospholipid molecular species and free fatty acids to analyse the lipid-binding ability and specificity of the allergen. Molecular modelling of the allergen was used to determine the potential regions involved in lipid interaction. Changes in Ole e 7 structure after lipid interaction were analysed by circular dichroism. Changes in the IgE binding upon ligand interaction were determined by ELISA. Wilhelmy balance measurements and fluorescence surfactant adsorption tests were performed to analyse the surface activity of the allergen. Using these different approaches, we have demonstrated the ability of Ole e 7 to interact and bind to a wide range of lipids, especially negatively charged phospholipids and oleic acid. We have also identified the protein structural regions and the residues potentially involved in that interaction, suggesting how lipid-protein interactions could define the behaviour of the allergen once inhaled at the airways.


Item Type:Article
Uncontrolled Keywords:Aeroallergen; nsLTP; Oleic acid; Phospholipids; Interfacial activity; Pulmonary surfactant
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:60754
Deposited On:04 Jun 2020 10:53
Last Modified:05 Jun 2020 08:22

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