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Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging

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Santos, Harrison D. A. and Zabala Gutiérrez, Irene and Shen, Yingli and Lifante, José and Ximendes, Erving and Laurenti, Marco and Méndez González, Diego and Melle Hernández, Sonia and Gómez Calderón, Óscar and López Cabarcos, Enrique and Fernández Monsalve, Nuria and Chavez Coria, Irene and Lucena Agell, Daniel and Monge, Luis and Mackenzie, Mark D. and Marqués Hueso, José and Jones, Callum M. S. and Jacinto, Carlos and Rosal, Blanca, del and Kar, Ajoy K. and Rubio Retama, Jorge and Jaque García, Daniel (2020) Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging. Nature communications, 11 (2933). 12 p.. ISSN 2041-1723

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Official URL: https://doi.org/10.1038/s41467-020-16333-2




Abstract

Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm−2) and doses (<0.5 mg kg−1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.


Item Type:Article
Additional Information:

Received: 11 May 2019; Accepted: 24 April 2020;
Published: 10 June 2020

Uncontrolled Keywords:Fluorescence imaging; Nanoparticles
Subjects:Medical sciences > Optics > Geometrical and instumental optics
Medical sciences > Optics > Imaging systems
ID Code:60947
Deposited On:20 Jun 2020 16:25
Last Modified:22 Jun 2020 16:01

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