Universidad Complutense de Madrid
E-Prints Complutense

Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy

Impacto

Downloads

Downloads per month over past year



Cuñado Rodríguez, Nieves (2020) Surface Spreading Technique in Plant Meiocytes for Analysis of Synaptonemal Complex by Electron Microscopy. In Plant Meiosis: Methods and Protocols. Methods in Molecular Biology, 2061 . Humana Press, New York, pp. 181-196. ISBN 9781493998180

[img] PDF
Restringido a Repository staff only

525kB

Official URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-9512-7_5



Abstract

An improved method of preparing two-dimensional surface spreads of synaptonemal complexes (SCs) in higher plants for examination by electron microscopy is described. This protocol produces clear, well-spread preparations of SCs and unpaired axial cores from a range of meiotic prophase I stages (leptotene to pachytene) from meiocytes of different plant species. Synaptonemal complex (SC) analyses have been widely used in plant cytogenetic studies to address the process of meiotic chromosome synapses, because of the high-resolution allowed by electron microscopy. Although the real role of SC is still enigmatic, its presence and structural conservation in the vast majority of organisms reflect the importance of this protein structure in the meiotic process.


Item Type:Book Section
Uncontrolled Keywords:Meiosis, synaptonemal complex, surface spreading, silver staining, electron microscopy
Subjects:Medical sciences > Biology > Plant physiology
Medical sciences > Biology > Genetics
Medical sciences > Biology > Microbiology
ID Code:60965
Deposited On:16 Jun 2020 12:36
Last Modified:16 Jun 2020 12:36

Origin of downloads

Repository Staff Only: item control page