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Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L

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Vera-Villalobos, Hernán and Lunario-Delgado, Lizzeth and Pérez-Retamal, Diana and Román, Domingo and Leiva, Juan Carlos and Zamorano, Pedro and Mercado-Seguel, Ana and Gálvez, Anita S and Benito Jiménez, César and Wulff-Zottele, Cristián (2020) Sulfate nutrition improves short-term Al3+-stress tolerance in roots of Lolium perenne L. Plant Physiology and Biochemistry, 148 . pp. 103-113. ISSN 0981-9428

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Official URL: https://www.sciencedirect.com/science/article/pii/S0981942820300115



Abstract

Trivalent aluminum ions (Al3+) in acidic soils are a major constraint for crop productivity inhibiting root elongation and promoting cell death. Al3+-toxicity has adverse biochemical and physiological effects on plant root growth. Sulfur is an essential macronutrient assimilated from the soil in the form of sulfate. However, the implication of sulfate nutritional status in the modulation of short-term Al3+-tolerance mechanisms in plant roots has not been previously reported. Here, we evaluated the effects of increased sulfate supply on short-term Al3+-toxicity in roots of Lolium perenne, measuring Al, Ca, Mg and S uptake, lipid peroxidation, total SOD activity, and transcriptional levels of Cu/Zn and Fe-SOD genes. First, the nitrogen sulfur ratio (N/S) in the TF nutrient solutions used in this study were computed to confirm that L. perenne plants were grown in sulfate deficiency (120 μM), optimal supply (240 μM), or overdoses conditions (360 μM), without affecting dry root biomass. Sulfate supplementation (>240 μM, and N/S ratio < 16) played a significant protection to Al3+-stress that prevents morphological changes in root tips, inhibits lipid peroxidation and differentially up-regulates total SOD activity, due changes in SOD gene expression. The results support the importance of sulfate nutritional status, on plant tissue homeostasis, enhancing the physiological tolerance mechanisms modulating lipid peroxidation damage induced by short-term Al3+-toxicity.


Item Type:Article
Uncontrolled Keywords:Al3+-toxicity, sulfate nutrition, perennial ryegrass (Lolium perenne, superoxide dismutase
Subjects:Medical sciences > Biology > Plant physiology
Medical sciences > Biology > Genetics
ID Code:62337
Deposited On:29 Sep 2020 13:55
Last Modified:29 Sep 2020 13:55

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