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Phylogeography of organophosphate resistant ace alleles in spanish olive fruit fly populations: a mediterranean perspective in the global change context

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Lantero Bringas, Esther and Matallanas Peñas, Beatriz and Pascual, Susana and Ochando González, María Dolores and Callejas Hervás, Carmen (2020) Phylogeography of organophosphate resistant ace alleles in spanish olive fruit fly populations: a mediterranean perspective in the global change context. Insects, 11 (6). pp. 1-12. ISSN ESSN 2075-4450

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Official URL: https://doi.org/10.3390/insects11060396




Abstract

The olive fruit fly (olf) Bactrocera oleae is the most damaging olive pest. The intensive use of organophosphates (OPs) to control it, led to an increase in resistance in field populations. This study assesses the presence and distribution of three mutations at the ace gene related to target site insensitivity to OPs in Spain. Samples from other Mediterranean countries were included as external references. Resistance-conferring alleles (from exons IV and VII of the ace gene) reached almost an 80% frequency in olf Spanish populations. In total, 62% of them were homozygous (RR/RR), this being more common in eastern mainland Spain. High frequencies of RR/RR individuals were also found in North Mediterranean samples. Conversely, in Tunisia, only sensitive alleles were detected. Finally, the exon X mutation ∆Q3 had an extremely low frequency in all samples. The high frequency of genotype RR/RR in Spain indicates high fitness in an agroecosystem treated with pesticides, in contrast to ∆Q3. At exon IV all flies carried the same haplotype for the allele conferring resistance. The sequence analysis at this exon suggests a unique origin and fast expansion of the resistant allele. These results provide evidence that OPs appropriate use is needed and prompt the search for alternative methods for olf pest control.


Item Type:Article
Uncontrolled Keywords:Bactrocera oleae (olf); Insecticide resistance; Organophosphates; Ace gene; Phylogeography; Exon IV; Exon VII; Exon X ∆Q3
Subjects:Medical sciences > Biology > Genetics
Medical sciences > Biology > Insects
ID Code:62426
Deposited On:07 Oct 2020 08:06
Last Modified:07 Oct 2020 09:52

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