Publication: Estudio de la capacidad volumétrica de la cámara pulpar de dientes temporales en relación con el aislamiento de células madre
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2020
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Abstract
En el año 2003, Miura y cols. demostraron la existencia de células madre en el tejido pulpar de dientes temporales. Desde entonces el número de artículos de investigación destinados a entender su aprovechamiento biológico ha aumentado exponencialmente. Sin embargo, tras la realización de una exhaustiva revisión bibliográfica, se observan lagunas en el proceso básico de recolección muestral.
El objetivo principal de este proyecto de investigación es determinar la capacidad volumétrica de la cámara pulpar de incisivos, caninos y molares temporales exfoliados naturalmente o exodonciados próximos al momento de exfoliación por presentar alguna alteración eruptiva el sucesor permanente. Para la realización del proyecto, se han recogido un total de 50 dientes, ausentes de patología inflamatoria o infecciosa. Para el análisis tridimensional de la muestra se ha recurrido al uso del CBCT y el software de reconstrucción volumétrica Slicer 4.10.2®.
Se intenta esclarecer cuál de estos tipos dentales es susceptible de contener una mayor cantidad de tejido pulpar remanente y, por tanto, su idoneidad para llevar a cabo el proceso básico de recolección y aislamiento celular. La capacidad volumétrica media de los distintos grupos dentales nos servirá como referencia para establecer un cribado en el proceso de selección muestral y aprovechamiento biológico de dichos dientes temporales.
In 2003, Miura et al. proved the existence of stem cells in the pulp tissue of temporary teeth. Since, the number of research articles aimed at understanding their biological use has increased exponentially. However, after carry out an exhaustive literature review, gaps in the basic sample collection process are observed. The main objective of this research is to determine the volumetric capacity of the pulp chamber of temporal incisors, canines and molars. Total of 50 teeth exfoliated or exodontized by eruptive alterations of the permanent successor have been collected without any pathology. For three-dimensional analysis of the sample, CBCT and volumetric reconstruction software Slicer 4.10.2® have been used. An attempt is made to clarify which of these dental types may contain a greater remaining pulp tissue, therefore, its suitability to carry out the basic process of cell collection and isolation. The volumetric capacity average of different dental groups will serve as a reference to establish a screening in the process of selection and biological use of naturally exfoliated deciduous teeth.
In 2003, Miura et al. proved the existence of stem cells in the pulp tissue of temporary teeth. Since, the number of research articles aimed at understanding their biological use has increased exponentially. However, after carry out an exhaustive literature review, gaps in the basic sample collection process are observed. The main objective of this research is to determine the volumetric capacity of the pulp chamber of temporal incisors, canines and molars. Total of 50 teeth exfoliated or exodontized by eruptive alterations of the permanent successor have been collected without any pathology. For three-dimensional analysis of the sample, CBCT and volumetric reconstruction software Slicer 4.10.2® have been used. An attempt is made to clarify which of these dental types may contain a greater remaining pulp tissue, therefore, its suitability to carry out the basic process of cell collection and isolation. The volumetric capacity average of different dental groups will serve as a reference to establish a screening in the process of selection and biological use of naturally exfoliated deciduous teeth.
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Encuadrado en la línea de investigación Normalidad y patología odontopediátrica. Determinación de protocolos terapéuticos y evaluación de su aplicabilidad en la Odontopediatría
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