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Sanz, A. Belén and García, Raúl and Rodríguez-Peña, José Manuel and Díez-Muñiz, Sonia and Nombela, César and Peterson, Craig L. and Arroyo, Javier (2012) Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway. Molecular biology of the cell, 23 (14). pp. 2805-2817. ISSN 1939-4586
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Official URL: http://www.molbiolcell.org/content/23/14/2805
Abstract
In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.
Item Type: | Article |
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Uncontrolled Keywords: | Microbiología |
Subjects: | Medical sciences > Pharmacy > Microbiology |
ID Code: | 21895 |
Deposited On: | 24 Jun 2013 13:38 |
Last Modified: | 25 May 2015 10:23 |
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