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Sub-proteomic study on macrophage response to Candida albicans unravels new proteins involved in the host defense against the fungus.

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Reales Calderón, Jose Antonio and Martínez Solano, Laura and Martínez Gomariz, Montserrat and Nombela, César and Molero, Gloria and Gil, Concha (2012) Sub-proteomic study on macrophage response to Candida albicans unravels new proteins involved in the host defense against the fungus. Journal of proteomics, 75 (15). pp. 4734-4746. ISSN 1876-7737

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Official URL: http://www.sciencedirect.com/science/article/pii/S1874391912000772



Abstract

In previous proteomic studies on the response of murine macrophages against Candida albicans, many differentially expressed proteins involved in processes like inflammation, cytoskeletal rearrangement, stress response and metabolism were identified. In order to look for proteins important for the macrophage response, but in a lower concentration in the cell, 3 sub-cellular extracts were analyzed: cytosol, organelle/membrane and nucleus enriched fractions from RAW 264.7 macrophages exposed or not to C. albicans SC5314 for 3 h. The samples were studied using DIGE technology, and 17 new differentially expressed proteins were identified. This sub-cellular fractionation permitted the identification of 2 mitochondrion proteins, a membrane receptor, Galectin-3, and some ER related proteins, that are not easily detected in total cell extracts. Besides, the study of different fractions allowed us to detect, not only total increase in Galectin-3 protein amount, but its distinct allocation along the interaction. The identified proteins are involved in the pro-inflammatory and oxidative responses, immune response, unfolded protein response and apoptosis. Some of these processes increase the host response and others could be the effect of C. albicans resistance to phagocytosis. Thus, the sub-proteomic approach has been a very useful tool to identify new proteins involved in macrophage-fungus interaction. This article is part of a Special Issue entitled: Translational Proteomics.


Item Type:Article
Uncontrolled Keywords:Microbiología
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:21904
Deposited On:24 Jun 2013 14:05
Last Modified:21 Jul 2015 08:47

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