Publication: Optical image processing to determine the optical properties of cellular membranes
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1984
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The Optical Society of America
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Abstract
In this paper we present a theoretical model for the transmission function of a biological cell. Three different cases are considered: real transmission function, pure phase object, general case, where both real and complex terms are present in the transmission function. In all cases we studied the response in the Fourier plane. Comparison of the proposed models leads us to conclude that the most correct treatment would come from the general case, where it was found that the membrane contribution predominates in the total diffracted amplitude distribution.
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© 1984 Optical Society of America.
International Optical Computing Conference (10ª. 1983. Cambridge, Massacghusets, EE.UU.).
This work was presented at the Tenth IOCC-ICO Congress, Cambridge, Mass., April 1983. It was partially financially supported by the Spanish Advisory Commission for Scientific and Technical Research under grant 4219/79.
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1. M. Kerker, The Scattering of Light and other Electromagnetic Radiation (Academic, New York, 1969).
2. A. Ishimaru, Wave Propagation and Scattering in Random Media (Academic, New York, 1978).
3. R. A. Meyer, Appl. Opt. 16, 2036 (1977).
4. N. Catsimpoolas, Ed., Cell Analysis, Vol. 1 (Plenum, New York, 1982).
5. J. W. Goodman, Introduction to Fourier Optics (McGraw-Hill, New York, 1968).