Endocytic mechanisms of graphene oxide nanosheets in 2 osteoblasts, hepatocytes and macrophages



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Linares, J. and Matesanz Sancho, Mª Concepción and Vila, Mercedes and Feito Castellano, María José and Vallet Regí, María and Marques, Paula A. and Portolés Pérez, Teresa (2014) Endocytic mechanisms of graphene oxide nanosheets in 2 osteoblasts, hepatocytes and macrophages. ACS Appl. Mater. Interfaces, 6 (16). pp. 13697-13706. ISSN 1944-8244

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Official URL: http://dx.doi.org/10.1021/am5031598


Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, henylarsine oxide and chlorpromazine) that specifically affect ifferent endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, cropynocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropynocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

Item Type:Article
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Subjects:Sciences > Chemistry > Chemistry, Inorganic
ID Code:30903
Deposited On:03 Jul 2015 10:36
Last Modified:20 Jul 2015 08:20

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