Publication: 1,3-Propanediol production from glycerol with a novel biocatalyst Shimwellia
blattae ATCC 33430: Operational conditions and kinetics in batch cultivations
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Publication Date
2015-10-23
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Elsevier
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Abstract
Shimwellia blattae ATCC 33430 as biocatalyst in the conversion of 1,3-propanediol from glycerol is herein evaluated. Several operational conditions in batch cultivations, employing pure and raw glycerol as sole carbon source, were studied. Temperature was studied at shaken bottle scale, while pH control strategy, together with the influence of raw glycerol and its impurities during fermentation were studied employing a 2 L STBR. Thereafter, fluid dynamic conditions were considered by changing the stirring speed and the gas supply (air or nitrogen) in the same scale-up experiments. The best results were obtained at a termperature of 37ºC, an agitation rate of 200 rpm, with free pH evolution from 6.9 and subsequent control at 6.5 and no gas supply during the fermentation, employing an initial concentration of 30 g/L of raw glycerol. Under these conditions, the biocatalyst is competitive, leading to results in line with other previous works in the literature in batch conditions, reaching a final concentration of 1,3-propanediol of 13.84 g/L, with a yield of 0.45 g/g and a productivity of 1.19 g/(L·h) from
raw glycerol.