Publication: Use of Raman spectroscopy for the early detection of
filaggrin-related atopic dermatitis
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Publication Date
2011-02
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Wiley
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Abstract
Background
Filaggrin (FLG) gene mutations, which result in complete or incomplete loss of proFLG/FLG peptides, have been reported as an important predisposing factor for atopic dermatitis (AD) and secondary atopic phenotypes such as atopic asthma.
Method
The presence of the protein FLG in the skin was evaluated at birth on 12 infants using Raman spectroscopy; these 12 infants were monitored for 1 year to see whether they developed AD. Three different statistical analysis procedures, two of which involved principal component analysis (PCA), were performed on the Raman spectra in order to determine the FLG content.
Results
The infants who had a lower FLG content, determined using any of the three statistical analysis procedures proposed, were also the ones that clinically developed AD.
Conclusion
The results suggest that Raman spectroscopy and statistical analysis such as PCA could be used as an early detection procedure for FLG -related AD and as a possible quantitative marker for FLG gene mutations
Description
"This is the pre-peer reviewed version of the following article, which has been published in final form at [https://doi.dx.org/10.1111/j.1600-0846.2010.00461.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving"
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1. Kezic S, Kemperman PMJH, Koster ES et al. Loss-offunction mutations in the filaggrin gene lead to reduced level of natural moisturizing factor in the stratum corneum. J Invest Dermatol 2008; 128: 2117–2119.
2. Nemoto-Hasebe I, Akiyama M, Nomura T, Sandilands A, McLean WHI, Shimizu H. Clinical severity correlates with impaired barrier in filaggrin-related eczema. J Invest Dermatol 2009; 129: 682–689.
3. Weidinger S, Illig T, Baurecht H et al. Loss-of-function variations within the filaggrin gene predispose for atopic dermatitis with allergic sensitizations. J Allergy Clin Immunol 2006; 118: 214–219.
4. Jollifee IT. Principal component analysis, 2nd edn. New York: Springer-Verlag, 2002.
5. Krafft C, Steiner G, Beleites C, Salzer R. Disease recognition by infrared and Raman spectroscopy. J Biophoton 2009; 2: 13–28.
6. Hannifin JM, Rajka G. Diagnostic features of atopic dermatitis. Acta Derm Venereol 1980; 92: 44–47.
7. Williams HC et al. The U.K. Working Party’s diagnostic criteria for atopic dermatitis. I. Derivation of a mínimum set of discriminators for atopic dermatitis. Br J Dermatol Q11994; 131: 383–396.
8. Morrison DF. Multivariate statistical methods, 3rd edn. Singapore: McGraw-Hill, 1990.
9. Rothe H, Duparr A, Jacobs S. Generic detrending of surface profiles. Opt Eng 1994; 33: 3023–3030.
10. Lopez-Alonso JM, Alda J, Bernabeu E. Principal component characterization of noise for infrared images. Appl Opt 2002; 41: 320–331.
11. Lopez-Alonso JM, Alda J. Operational parametrization of the 1/f noise of a sequence of frames by means of the principal component analysis in focal plane arrays. Opt Eng 2004; 42: 1915–1922.