Publication: Detección molecular de micrometástasis en ganglio centinela : valor pronóstico
Loading...
Files
Official URL
Full text at PDC
Publication Date
2016-03-15
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
El cáncer de mama es el segundo cáncer más frecuente en el mundo y el tumor maligno más frecuente entre las mujeres. Cambios en las células tumorales junto con el microambiente tumoral facilitan el desprendimiento de células tumorales de la masa primaria del tumor. Cualquier nodo que reciba directamente el drenaje linfático del tumor primario será el ganglio centinela GC, que se convierte en el primer nodo involucrado en el proceso de metastatización de un tumor vía sistema linfático. Durante años las pacientes han sido sometidas a la disección axilar de la cadena ganglionar completa para evitar que estas células se diseminaran al resto del organismo, pero a la vez, esta intervención provoca una importante morbilidad. La técnica de la biopsia del ganglio centinela (BGC) surge de la idea de que la diseminación de células tumorales a través de los ganglios linfáticos se produce de manera ordenada, de modo que la ausencia de células tumorales metastásicas en el GC predice, de forma precisa, la ausencia de metástasis en los nodos axilares restantes.. Los GC extraídos se analizan por medio de tinción con hematoxilina-eosina (H&E) y por tinción inmunohistoquímica (IHQ). Se ha demostrado que con estas técnicas se produce un número importante de falsos negativos, quedando metástasis ocultas sin identificar...
Breast cancer is the second most frequent cancer worldwide, and the most frequent among women. Specific molecular changes occurring within the tumor cells and tumor microenvironment contribute to the detachment of tumor cells from the primary tumor mass. Any lymph node receiving direct lymphatic drainage from the primary tumor is the sentinel lymph node (SLN), becoming the first node involved when a tumor metastatizes via lymphatics. To avoid tumor cells dissemination, axillary lymph node dissection (ALND) has been used for many years, although the procedure carries important morbidity. Sentinel lymph node biopsy (SLNB) arises from the idea that lymphatic metastases occur in an orderly manner, thus the absence of metastatic tumor cells in the SLN accurately predicts the absence of metastases in the remaining axillary nodes. Resected SLN are stained with hematoxylin and eosin (H&E) and with immunohistochemistry (IHC). It has been stated that these procedures give place to an important number of false negative results, remaining occult metastases undetected. In the last decade molecular biology techniques have been developed focused on the molecular detection of micrometastases in SLN, like the retro transcriptasepolymerase chain reaction (RT-PCR). This technology detects the expression of tumor markers in tumor cells, that are not expressed, or they do in low proportion, in healthy tissue...
Breast cancer is the second most frequent cancer worldwide, and the most frequent among women. Specific molecular changes occurring within the tumor cells and tumor microenvironment contribute to the detachment of tumor cells from the primary tumor mass. Any lymph node receiving direct lymphatic drainage from the primary tumor is the sentinel lymph node (SLN), becoming the first node involved when a tumor metastatizes via lymphatics. To avoid tumor cells dissemination, axillary lymph node dissection (ALND) has been used for many years, although the procedure carries important morbidity. Sentinel lymph node biopsy (SLNB) arises from the idea that lymphatic metastases occur in an orderly manner, thus the absence of metastatic tumor cells in the SLN accurately predicts the absence of metastases in the remaining axillary nodes. Resected SLN are stained with hematoxylin and eosin (H&E) and with immunohistochemistry (IHC). It has been stated that these procedures give place to an important number of false negative results, remaining occult metastases undetected. In the last decade molecular biology techniques have been developed focused on the molecular detection of micrometastases in SLN, like the retro transcriptasepolymerase chain reaction (RT-PCR). This technology detects the expression of tumor markers in tumor cells, that are not expressed, or they do in low proportion, in healthy tissue...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Departamento de Bioquímica y Biología Molecular I, leída el 26-11-2015