Publication: Modificación del patrón de expresión genética de células madre mesenquimales de origen de pulpa dental humana mediante medio de diferenciación comercial y medio condicionado
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2016-04-21
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Universidad Complutense de Madrid
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Abstract
El término de célula madre hace referencia a un tipo de célula que es capaz de diferenciarse a distintos tipos de células. En realidad, por motivos técnicos no se experimenta con las mejores células madre que hay que son las embrionarias y que dada su pluripotencialidad, podrían dar lugar a cualquier tipo de célula de un organismo. Las células madre que tenemos a nuestro alcance son las del adulto o postnatales y son multipotentes, diferenciándose a linajes específicos. Dentro de este grupo, se encuentran las células madre mesenquimales (SCs), que se diferencian a los 3 linajes mesodérmicos: osteoblastos, adipocitos y condrocitos. Dentro del campo odontológico, hay una fuente muy accessible de SCs: la pulpa dental (DPSC). Son de apariencia más fibroblastoide, pueden ser criopreservadas, proliferan extensamente (hasta 25 pases), son poco inmunogénicas (no producen tanta inflamación) expresan marcadores mesenquimales y tienen mayor plasticidad. La caracterización de las DPSC en gran medida se basa en el uso de métodos tales como tinciones histoquímicas, marcaje inmune por citometría de flujo y transcripción inversa PCR (reacción en cadena de la polimerasa) para establecer tanto la identidad de células madre como la función...
The term stem cell refers to a cell type that is able to differentiate into different cell types. Actually, for technical reasons, experiments are not conducted with the best stem cells available, the embryonic cells. Due to the pluripotency of these cells, they could result in any type of cellsof an organism. We have at our disposal adult or postnatal stem cells. These are only multipotent, and can be differentiated into specific lineages. Within this group there are mesenchymal stem cells (SCs) which differentiate into three mesodermal lineages: osteoblasts, adipocytes and chondrocytes. Within the dental field, there is a very accessible source of stem cells: the dental pulp (DPSC). They have a more fibroblastoid appearance, can be cryopreserved, proliferate extensively (up to 25 passes), are less immunogenic (they produce less inflammation), express mesenchymal markers and have greater plasticity. The characterization of the DPSCs is based largely on the use of methods such as histochemical staining, flow cytometry and reverse transcription by PCR (Polymerase Chain Reaction) to establish both the identity and function of stem cells...
The term stem cell refers to a cell type that is able to differentiate into different cell types. Actually, for technical reasons, experiments are not conducted with the best stem cells available, the embryonic cells. Due to the pluripotency of these cells, they could result in any type of cellsof an organism. We have at our disposal adult or postnatal stem cells. These are only multipotent, and can be differentiated into specific lineages. Within this group there are mesenchymal stem cells (SCs) which differentiate into three mesodermal lineages: osteoblasts, adipocytes and chondrocytes. Within the dental field, there is a very accessible source of stem cells: the dental pulp (DPSC). They have a more fibroblastoid appearance, can be cryopreserved, proliferate extensively (up to 25 passes), are less immunogenic (they produce less inflammation), express mesenchymal markers and have greater plasticity. The characterization of the DPSCs is based largely on the use of methods such as histochemical staining, flow cytometry and reverse transcription by PCR (Polymerase Chain Reaction) to establish both the identity and function of stem cells...
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Tesis inédita de la Universidad Complutense de Madrid de la Facultad de Odontología del Departamento de Estomatología III, leída el 20-01-2016