ArmA methyltransferase in a monophasic Salmonella enterica isolate from food



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Granier, Sophie A and Hidalgo, Laura and San Millán, Álvaro and Escudero, José Antonio and Gutiérrez, Belén and Brisabois, Anne and González Zorn, Bruno (2011) ArmA methyltransferase in a monophasic Salmonella enterica isolate from food. Antimicrobial agents and chemotherapy, 55 (11). pp. 5262-6. ISSN 1098-6596

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The 16S rRNA methyltransferase ArmA is a worldwide emerging determinant that confers high-level resistance to most clinically relevant aminoglycosides. We report here the identification and characterization of a multidrug-resistant Salmonella enterica subspecies I.4,12:i:- isolate recovered from chicken meat sampled in a supermarket on February 2009 in La Reunion, a French island in the Indian Ocean. Susceptibility testing showed an unusually high-level resistance to gentamicin, as well as to ampicillin, expanded-spectrum cephalosporins and amoxicillin-clavulanate. Molecular analysis of the 16S rRNA methyltransferases revealed presence of the armA gene, together with bla(TEM-1), bla(CMY-2), and bla(CTX-M-3). All of these genes could be transferred en bloc through conjugation into Escherichia coli at a frequency of 10(-5) CFU/donor. Replicon typing and S1 pulsed-field gel electrophoresis revealed that the armA gene was borne on an ~150-kb broad-host-range IncP plasmid, pB1010. To elucidate how armA had integrated in pB1010, a PCR mapping strategy was developed for Tn1548, the genetic platform for armA. The gene was embedded in a Tn1548-like structure, albeit with a deletion of the macrolide resistance genes, and an IS26 was inserted within the mel gene. To our knowledge, this is the first report of ArmA methyltransferase in food, showing a novel route of transmission for this resistance determinant. Further surveillance in food-borne bacteria will be crucial to determine the role of food in the spread of 16S rRNA methyltransferase genes worldwide.

Item Type:Article
Subjects:Medical sciences > Veterinary
ID Code:39678
Deposited On:09 Jan 2017 16:07
Last Modified:09 Jan 2017 16:07

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