Macroglial and retinal changes in hypercholesterolemic rabbits after normalization of cholesterol levels



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Ramírez Sebastián, Ana Isabel and Salazar Corral, Juan José and Hoz Montañana, María Rosa de and Rojas López, María Blanca and Ruiz, Emilio and Tejerina Sánchez, Teresa and Ramirez Sebastian, Jose Manuel and Triviño Casado, Alberto (2006) Macroglial and retinal changes in hypercholesterolemic rabbits after normalization of cholesterol levels. Experimental Eye Research, 83 (6). pp. 1423-1438. ISSN 0014-4835

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This study evaluates hypercholesterolemic rabbits, examining the retinal changes in Müller cells and astrocytes as well as their variations after a period of normal blood-cholesterol values induced by a standard diet. New Zealand rabbits were divided into three groups: G0, fed a standard diet; G1A, fed a 0.5% cholesterol-enriched diet for 8 months; and G1B, fed as G1A followed by standard diet for 6 months. Eyes were processed for transmission electron microscopy and immunohistochemistry (GFAP). While G1B resembled G0 more than did G1A, they shared alterations with G1A: a) as in G1A, Müller cells were GFAP+, filled spaces left by axonal degeneration, formed glial scars and their nuclei were displaced to the nerve-fibre layer. The area occupied by the astrocytes associated with the nerve-fibre bundles (AANFB) and by perivascular astrocytes (PVA) in G1A and G1B was significantly lower than in controls. However, no significant differences in PVA were found between G1A and G1B. In G1B, type I PVA was absent and replaced by hypertrophic type II cells; b) Bruch's membrane (BM) was thinner in G1B than in G1A; c) the retinal pigment epithelium (RPE) cytoplasm contained fewer lipids in G1B than in G1A; d) in G1A and G1B choriocapillaris and retinal vessel showed alterations with respect to G0; e) cell death and axonal degeneration in the retina were similar in G1A and G1B. The substitution of a hyperlipemic diet by a standard one normalizes blood-lipid levels. However, the persistence of damage at retinal vessels and BM-RPE could trigger chronic ischemia.

Item Type:Article
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Received 4 May 2006; accepted in revised form 22 July 2006
Available online 26 September 2006

Uncontrolled Keywords:cholesterol-fed rabbit; retina; electron microscopy; Immunohistochemistry; GFAP; Bruch’s membrane; astrocytes; Müller cells
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Biology > Neurosciences
Medical sciences > Optics > Eyes anatomy
ID Code:40474
Deposited On:05 Dec 2016 10:51
Last Modified:05 Dec 2016 10:51

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