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Viña Fernández, Eliseo and Parisi, Valentina and Sanchez Ramos, Celia and Cabo, Roberto and Guerrera, María Cristina and Quirós, Luis Manuel and Germaná, Antonio M. and Vega, José Antonio and García Suárez, Olivia (2015) Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish. Cell and Tissue Research, 360 (2). pp. 223-231. ISSN 0302-766X
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Official URL: http://dx.doi.org/10.1007/s00441-014-2084-5
Abstract
Acid-sensing ion channels (ASICs) are H+-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs.
Item Type: | Article |
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Additional Information: | The online version of this article contains supplementary material, which is available to authorized users. |
Uncontrolled Keywords: | Retina, Acid-sensing ion channels, Light, Zebrafish |
Subjects: | Medical sciences > Biology > Cytology Medical sciences > Biology > Animal physiology Medical sciences > Optics > Physiological optics |
ID Code: | 40842 |
Deposited On: | 25 Jan 2017 11:46 |
Last Modified: | 25 Jan 2017 11:46 |
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