Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites



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Matesanz Sancho, María Concepción and Feito Castellano, María José and Oñaderra Sánchez, Mercedes and Ramirez Santillán, Cecilia and Casa, Carmen da and Arcos Navarrete, Daniel and Vallet Regí, María and Rojo, José María and Portolés Pérez, María Teresa (2014) Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites. Journal of Colloid and Interface Science, 416 . pp. 59-66. ISSN 0021-9797

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Official URL: https://www.elsevier.com/


Hypothesis: Synthetic hydroxyapatite (HA) and Si substituted hydroxyapatite (SiHA) are calcium phosphate ceramics currently used in the field of dentistry and orthopaedic surgery. The preparation of both biomaterials as polycrystalline solid pieces or grains formed by nanocrystallites has awakened a great interest to enhance the bioactive behavior due to the microstructural defects and the higher surface area. The study of the macrophage and lymphocyte behavior in contact with nanocrystalline HA and SiHA will allow to elucidate the immune response which conditions the success or rejection of these biomaterials.

Experiments: HA and SiHA granules (with sizes of tens of microns) have been prepared by controlled aqueous precipitation avoiding subsequent high temperature sintering. HA and SiHA granules were constituted by crystallites smaller than 50 nm. The effects of both nanocrystalline materials on immune system have been evaluated with macrophages (main components of innate immune system) and T lymphocytes (specific cells of adaptive response) after short-term culture as in vitro models of the early immune response.

Findings: Significant decreases of macrophage proliferation and phagocytic activity, increased production of inflammatory cytokines (IL-6, TNF-a) and T lymphocyte apoptosis, were induced by these nanocrystalline ceramics suggesting that, after in vivo implantation, they induce significant effects on immune responses, including an early activation of the innate immune system. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Biocompatibility; Cytokine; Hydroxyapatite; Lymphocyte; Macrophage, Silicon-substituted hydroxyapatite; Mesoporous bioactive glass; Antigen-presenting cells; Immune-responses; Calcium; Biomaterials; Bioceramics; Osteoblasts; Biocompatibility; Fibroblasts
Subjects:Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:40872
Deposited On:01 Feb 2017 13:09
Last Modified:03 Feb 2017 12:27

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