Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2



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Torres-Rodriguez, Carolina and Portolés Pérez, M. T. and Matesanz Sancho, María Concepción and Linares, Javier and Feito Castellano, María José and Izquierdo-Barba, Isabel and Esbrit, Pedro and Vallet Regí, María (2016) Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2. Journal of Colloid and Interface Science, 461 . pp. 285-291. ISSN 0021-9797

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Official URL: https://www.elsevier.com/


Hypothesis: Dental bleaching with H2O2 is a common daily practice in dentistry to correct discoloration of anterior teeth. The aim of this study has been to determine whether this treatment of human teeth affects growth, differentiation and activity of osteoclast-like cells, as well as the putative modulatory action of osteostatin and fibroblast growth factor 2 (FGF-2).

Experiments: Previously to the in vitro assays, structural, physical-chemical and morphological features of teeth after bleaching were studied. Osteoclast-like cells were cultured on human dentin disks, pre-treated or not with 38% H2O2 bleaching gel, in the presence or absence of osteostatin (100 nM) or FGF-2 (1 ng/ml). Cell proliferation and viability, intracellular content of reactive oxygen species (ROS), pro-inflammatory cytokine (IL-6 and TNF alpha) secretion and resorption activity were evaluated.

Findings: Bleaching treatment failed to affect either the structural or the chemical features of both enamel and dentin, except for slight morphological changes, increased porosity in the most superficial parts (enamel), and a moderate increase in the wettability degree. In this scenario, bleaching produced an increased osteoclast-like cell proliferation but decreased cell viability and cytokine secretion, while it augmented resorption activity on dentin. The presence of either osteostatin or FGF-2 reduced the osteoclast-like cell proliferation induced by bleaching. FGF-2 enhanced ROS content, whereas osteostatin decreased ROS but increased TNF alpha secretion. The bleaching effect on resorption activity was increased by osteostatin, but this effect was less evident with FGF-2.

Conclusions: These findings further confirm the deleterious effects of tooth bleaching by affecting osteoclast growth and function as well as different modulatory actions of osteostatin and FGF-2. (C) 2015 Elsevier Inc. All rights reserved.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Dental bleaching, External cervical resorption, Osteoclast, Fibroblast growth factor 2, Osteostatin
Subjects:Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Inorganic
ID Code:41096
Deposited On:09 Feb 2017 12:13
Last Modified:13 Sep 2017 23:01

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